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KMID : 0608520060120030059
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Korean Journal of Oriental Medicine
2006 Volume.12 No. 3 p.59 ~ p.67
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Effects of electroacupuncture on the expression of iNOS in Nuclei of solitary tract and Rostral ventrolateral medulla in spontaneously hypertensive rats
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Hwang Hye-suk
Kim Yoo-Sung
Lee ji-Eun
Han Kyung-Ju
Choi Sun-Mi
Koo Sung-Tae
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Abstract
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Aims: Acupuncture has been used for the treatment of essential hypertension, but the efficacy and the mechanism of acupuncture in prevention of hypertension are still unclear. We tested the hypothesis that electroacupuncture (EA) applied to Baekhoe (GV20) changes NO/NOS system during development of hypertension in spontaneously hypertensive rats (SHR) , and thereby causes the delay of development of hypertension in SHR.
Methods: The male SHR rats in the developmental stages of hypertension (7-8 weeks) were randomly divided into three groups (control group, GV20 acupuncture group, and tail acupuncture group). And the age matched Wistar Koyto Rats (WKY) were randomly divided into two groups (negative control, GV20 acupuncture group). EA treatments (10Hz, 1mA, 0.1ms) were carried out for 25 mini day for five consecutive days. The systolic blood pressure (SBP) was determined in conscious rats by the tail-cuff method using automatic BP mornitoring system. We investigated the activations of inducible NO synthase (iNOS) in nuclei of solitary tract (NTS) and rostral ventrolateral medulla (RVLM) of SHR by the western blotting method.
Results: The SBP after the termination of EA stimulation applied to the GV20 was stabilized at 169.14±3.67 ㎜Hg which is lower value than that of the control group. The SBP of control group was elevated to 178.14±3.49 ㎜Hg. In addition, we evaluated NOS activity as well as iNOS protein expression of NTS and RVLM in both of SHR and WKY. The iNOS activity in NTS was significantly higher in SHR than in WKY. Furthermore, the iNOS activity of NTS showed significant decreases in EA groups compare to that of non treated SHR group. Although iNCS expression of RVLM showed non significant changes between SHR and WKY, EA significantly enhanced the iNCS expression in SHR. Our data support the hypothesis that delayed development of hypertension and altered iNOS expression of NTS and RVLM by EA stimulations in SHR rats.
Conclusions: The findings demonstrate that acupuncture can change NO/NCS system in NTS and RVLM, and exert beneficial role on development of hypertension.
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KEYWORD
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Hypertension, Spontaneously hypertensive rat, GV20, Nuclei of solitary tract, Rostral ventrolateral medulla
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